Generator

Part:BBa_J119304

Designed by: Todd Eckdahl, Claire Shinneman   Group: Eckdahl Lab   (2013-06-10)

eCDM8 expression with low promoter and low C dog RBS

A low promoter, J119030 with a low c.dog J119025, this combination is known as S05011. The eCDM8 gene (J100100) was then inserted into the S05011 vector creating a pSB1A8 plasmid with a low promoter, low c.dog, and the eCDM8 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 199
    Illegal AgeI site found at 860
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None